This study evaluated an intact cell dissociation method for formalin-fixed paraffin-embedded (FFPE) colon tissue to improve cell recovery and gene detection in single-cell RNA sequencing (scRNA-seq), compared to standard dissociation approaches.
The intact cell dissociation protocol enhanced both cell recovery and gene detection in scRNA-seq from FFPE colon tissue, suggesting improved transcriptomic profiling relative to conventional methods.
The abstract provides limited detail on sample size, comparator conditions, and whether findings generalize beyond colon tissue or to other FFPE tissue types.
When performing scRNA-seq on archival FFPE colon specimens, consider intact cell dissociation protocols to maximize cell yield and transcriptomic data quality. This may expand the utility of banked FFPE samples for single-cell studies.
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