## **Novel H5 recombinant protein vaccine provides effective protection against H5N1 influenza virus in cats** ## **Supplementary Table 1. Clinical signs used to calculate the clinical score index** | Days
post
challenge | Cat
ID | Hunched
posture | Ruffled fur
(piloerection) | Not eating
or drinking | >15% body
weight loss | Neurological
symptoms
(hind-limb
paralysis) | Total
score | |---------------------------|-----------|--------------------|-------------------------------|---------------------------|--------------------------|------------------------------------------------------|----------------| | | | ( 3 points) | ( 3 points) | (2 points) | (10 points) | (10 points) | 28 | | | C1 | 3 | 3 | 0 | 0 | 0 | 6 | | | C3 | 3 | 0 | 0 | 0 | 0 | 3 | | | C4 | 3 | 3 | 0 | 0 | 0 | 6 | | | C6 | 3 | 0 | 0 | 0 | 0 | 3 | | 1 | C7 | 3 | 0 | 0 | 0 | 0 | 3 | | | C8 | 3 | 3 | 0 | 0 | 0 | 6 | | | C11 | 3 | 3 | 0 | 0 | 0 | 6 | | | C16 | 3 | 3 | 0 | 0 | 0 | 6 | | | C1 | 3 | 3 | 0 | 0 | 0 | 6 | | | C3 | 3 | 3 | 0 | 0 | 0 | 6 | | | C4 | 3 | 3 | 2 | 0 | 0 | 8 | | 2 | C6 | 3 | 3 | 2 | 0 | 0 | 8 | | | C7 | 3 | 3 | 2 | 0 | 0 | 8 | | | C8 | 3 | 3 | 2 | 0 | 0 | 8 | | | C11 | 3 | 3 | 2 | 0 | 0 | 8 | | | C16 | 3 | 3 | 2 | 0 | 0 | 8 | | | C1 | 3 | 3 | 2 | 0 | 0 | 8 | | | C3 | 3 | 3 | 0 | 0 | 0 | 6 | | | C4 | 3 | 3 | 0 | 0 | 0 | 6 | | | C6 | 3 | 3 | 2 | 0 | 0 | 8 | | 3 | C7 | 3 | 3 | 2 | 0 | 0 | 8 | | | C8 | 3 | 3 | 2 | 0 | 0 | 8 | | | C11 | 3 | 3 | 0 | 0 | 0 | 6 | | | C16 | 3 | 3 | 2 | 0 | 0 | 8 | | | 1 | 3 | 3 | 2 | 0 | 0 | 8 | | | 3 | 3 | 3 | 0 | 0 | 0 | 6 | | | 4 | 3 | 3 | 2 | 0 | 0 | 8 | | | 6 | 3 | 3 | 0 | 0 | 0 | 6 | | | 7 | 3 | 3 | 2 | 0 | 0 | 8 | | 4 | 8 | 3 | 3 | 0 | 0 | 0 | 6 | | | 11 | 3 | 3 | 2 | 0 | 0 | 8 | | | 16 | 3 | 3 | 2 | 0 | 0 | 8 | | | 1 | 3 | 3 | 2 | 0 | 0 | 8 | |----|----|---|---|---|----|----|----| | 5 | 3 | 3 | 3 | 0 | 0 | 0 | 6 | | | 4 | 3 | 3 | 2 | 0 | 0 | 8 | | | 6 | 3 | 3 | 2 | 0 | 0 | 8 | | | 7 | 3 | 3 | 2 | 0 | 0 | 8 | | | 8 | 3 | 3 | 2 | 0 | 0 | 8 | | | 11 | 3 | 3 | 2 | 0 | 0 | 8 | | | 16 | 3 | 3 | 2 | 0 | 0 | 8 | | | 1 | 3 | 3 | 2 | 0 | 0 | 8 | | | 3 | 3 | 3 | 2 | 0 | 0 | 8 | | | 4 | 3 | 3 | 2 | 0 | 0 | 8 | | | 6 | 3 | 3 | 2 | 0 | 0 | 8 | | 6 | 7 | 3 | 3 | 2 | 0 | 0 | 8 | | | 8 | 3 | 3 | 2 | 10 | 0 | 18 | | | 11 | 3 | 3 | 2 | 10 | 0 | 18 | | | 16 | | | 2 | 10 | 0 | 12 | | 7 | 1 | 3 | 3 | 2 | 10 | 0 | 18 | | | 3 | 3 | 3 | 2 | 0 | 0 | 8 | | | 4 | 3 | 3 | 2 | 0 | 0 | 8 | | | 6 | 3 | 3 | 2 | 10 | 0 | 18 | | | 7 | 3 | 3 | 2 | 0 | 0 | 8 | | | 8 | 3 | 3 | 2 | 10 | 0 | 18 | | | 11 | 3 | 3 | 2 | 10 | 0 | 18 | | | 16 | 3 | 3 | 2 | 10 | 0 | 18 | | 8 | C1 | 3 | 3 | 2 | 10 | 0 | 18 | | | C3 | 3 | 3 | 2 | 0 | 0 | 8 | | | C4 | 3 | 3 | 2 | 10 | 0 | 18 | | | C7 | 3 | 3 | 2 | 10 | 10 | 28 | | | C8 | 3 | 3 | 2 | 10 | 0 | 18 | | | C1 | 3 | 3 | 2 | 10 | 0 | 18 | | | C3 | 3 | 3 | 0 | 0 | 0 | 6 | | 9 | C4 | 3 | 3 | 2 | 10 | 0 | 18 | | 10 | C3 | 3 | 3 | 0 | 0 | 0 | 6 | | 14 | C3 | 3 | 0 | 0 | 0 | 0 | 3 | ## **Supplementary Table 2. Nucleotide similarity of hemagglutinin gene sequences** | Isolates | H5-vaccine (A2) | First *HPAI H5N1 in #NA
(Unassigned) | Challenge (B3.13) | |-----------------------------------------|-----------------|-----------------------------------------|-------------------| | First *HPAI H5N1
in #NA (Unassigned) | 99.30 | | | | Challenge (B3.13) | 98.94 | 99.65 | | | Heterologous (D1.1) | 98.24 | 98.77 | 98.42 | ^# North America ^\* Highly pathogenic avian influenza