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Cells were pre-gated on single viable CD11b+F4/80+ (a, b ).0 500K 1.0M 1.5M 2.0M 0 500K 1.0M 1.5M 2.0M FSC-A 0 500K 1.0M 1.5M 2.0M 0 500K 1.0M 1.5M 2.0M 0 500K 1.0M 1.5M 2.0M FSC-A 0 500K 1.0M 1.5M 2.0M FSC-A ### Page 3 Supplementary Table 1. Transition list for metabolites measured with reversed phase and ion paired chromatography fortargeted metabolomics.RT, Retention TimeReversed Phase Transition ListName RT IonPolarityTransition CollisionEnergyFragmentor Transition CollisionEnergyFragmentor1-methylnicotinamide_3106-60-3_C02918 0,905 Positive 137.0 ->94.120 120 137.0 ->92.020 120S-Adenosyl-L-Methionine_485-80-3_C00019 0,906 Positive 399.2 ->250.016 120 399.2 ->136.032 120L-carnitine_541-15-1_C00318 0,911 Positive 162.1 ->60.220 120 162.1 ->103.020 120NicotinamideMononucleotide_1094-617_C004551,061 Positive 335.1 ->123.012 95 335.1 ->80.160 95NicotinicAcid_59-67-6_C00253 1,263 Positive 124.0 ->78.130 80 124.0 ->80.120 80Methionine_63-68-3_C00073 1,351 Positive 150.0 ->56.020 60 150.0 ->61.020 60O-Acetylcarnitine_3040-38-8_C02571 1,404 Positive 204.1 ->85.020 120 204.1 ->145.010 120QuinolinicAcid_89-00-9_C03722 1,454 Positive 168.0 ->78.024 82 168.0 ->124.08 82Nicotinamide_98-92-0_C00153 1,555 Positive 123.1 ->80.024 105 123.1 ->53.036 105OphthalmicAcid_495-27-2_C21016 1,589 Positive 290.1 ->58.220 80 290.1 ->215.010 80Nudifloramide_701-44-0_C05842 1,887 Positive 153.1 ->108.024 135 153.1 ->78.036 1353-Hyroxykynurenine_606-14-4_C03227 1,924 Positive 225.0 ->110.016 92 225.0 ->162.020 92NN-Dimethyltryptamine_61-50-7_C08302 2,349 Positive 189.1 ->189.10 82 189.1 ->144.110 82 ### Page 4 PicolinicAcid_98-98-6_C10164 2,450 Positive 124.0 ->78.020 82 124.0 ->106.08 82Propionylcarnitine_17298-37-2_C03017 3,132 Positive 218.1 ->159.010 120 218.1 ->144.120 120S-Adenosyl-L-Homocysteine_979-920_C000213,171 Positive 385.1 ->136.020 115 385.1 ->134.020 115N-Methyl-4-Pyridone-3-Carboxamide_76949-3_C058433,373 Positive 153.1 ->136.016 85 153.1 ->92.028 85N-methylNicotinamide_114-33-0_C05843 3,382 Positive 137.1 ->53.220 120 137.1 ->80.124 115Glutarylcarnitine_102636-82-8_NA 3,522 Positive 276.1 ->199.015 160 276.1 ->216.915 160Kynurenine_2922-83-0_C00328 4,103 Positive 209.0 ->209.00 92 209.0 ->146.020 92ISTD-D8-Tryptophan_NA_NA 4,614 Positive 212.0 ->195.010 50 212.0 ->150.020 80Methylmalonate_516-05-2_C02170 4,628 Positive 119.1 ->73.110 80 119.1 ->55.120 80Butyrylcarnitine_1492-26-8_C02862 4,662 Positive 232.1 ->57.145 120 232.1 ->144.120 120Isobutyrylcarnitine_25518-49-4_NA 4,670 Positive 232.1 ->85.020 120 232.1 ->173.010 120NN-Dimethylserotonin_487-93-4_C08299 4,761 Positive 205.0 ->205.00 80 205.0 ->184.15 80Tryptophan_73-22-3_C00078 4,761 Positive 205.0 ->146.016 82 205.0 ->118.032 82ISTD-13C11-Tryptophan_202114-65-6_NA 4,769 Positive 216.0 ->155.015 50 216.0 ->169.015 503-hydroxyanthranillic acid_548-93-6_C00632 4,821 Positive 154.0 ->108.020 114 154.0 ->154.00 114KynurenicAcid_492-27-3_C01717 4,900 Positive 190.0 ->144.020 104 190.0 ->116.036 104NicotinuricAcid_583-08-4_C05380 4,925 Positive 181.1 ->135.020 115 181.1 ->79.148 115 ### Page 5 Tryptamine_61-54-1_C00398 5,048 Positive 161.1 ->91.140 60 161.1 ->115.040 602-Methylbutyryl carnitine_31023-25-3_NA 5,075 Positive 246.0 ->60.220 120 246.0 ->144.120 120Isovalerylcarnitine_31023-24-2_C20826 5,075 Positive 246.1 ->187.015 80 246.1 ->85.120 80Serotonin_50-67-9_C00780 5,340 Positive 177.1 ->115.040 82 177.1 ->77.160 82NicotinamideN-oxide_1986-81-8_NA 5,350 Positive 139.0 ->78.036 139 139.0 ->106.024 139AnthranillicAcid_5326-47-6_NA 5,423 Positive 138.0 ->92.020 80 138.0 ->65.030 80N-methylTryptamine_61-49-4_C06213 5,462 Positive 175.1 ->132.012 80 175.1 ->144.012 80NicotinicAcidMononucleotide_321-028_C011855,481 Positive 337.1 ->125.016 95 337.1 ->97.020 95N-methylserotonin_1134-01-6_C06212 5,493 Positive 191.0 ->115.040 80 191.0 ->160.020 80Hexanoylcarnitine_22671-29-0_NA 5,530 Positive 260.2 ->201.015 80 260.2 ->99.120 120Octanoylcarnitine_25243-95-2_C02838 6,216 Positive 288.1 ->229.110 160 288.1 ->144.120 160Decanoylcarnitine_1492-27-9_C03299 6,756 Positive 316.2 ->257.120 160 316.2 ->155.220 160LipoicAcid_1200-22-2_C16241 7,046 Negative 205.0 ->127.015 150 205.0 ->171.05 100Lauroylcarnitine_25518-54-1_NA 7,147 Positive 344.3 ->285.220 160 344.3 ->183.020 200Myristoylcarnitine_25597-07-3_NA 7,595 Positive 372.3 ->313.220 200 372.3 ->229.220 200Palmitoylcarnitine_2364-67-2_C02990 7,929 Positive 400.2 ->95.030 200 400.2 ->341.220 160Oleoylcarnitine_38677-66-6_NA 7,986 Positive 426.0 ->85.030 100 426.0 ->144.030 50 ### Page 6 Stearoylcarnitine_25597-09-5_NA 8,288 Positive 428.3 ->267.120 120 428.3 ->369.220 120Ion Paired Transition ListName RT IonPolarityTransition CollisionEnergyFragmentor Transition CollisionEnergyFragmentorD-+-Galactosamine 1,096 Negative 238.1 ->159.98 70 238.1 ->100.06 70Thiamine 1,12 Negative 264.1 ->234.06 124Pyridoxamine 1,139 Negative 167.1 ->121.022 102 167.1 ->122.018 102L-Histidine 1,148 Negative 154.1 ->93.116 102 154.1 ->137.012 102L-asparagine 1,21 Negative 131.0 ->113.04 86 131.0 ->70.012 86L-Carnitine 1,214 Negative 220.1 ->146.04 66L-Cystathionine 1,214 Negative 221.1 ->134.08 92 221.1 ->120.09 92L-Serine 1,22 Negative 104.0 ->74.18 70S-2-Aminoethyl-L-cysteine 1,224 Negative 163.1 ->76.110 88 163.1 ->33.228 88L-Glutamine 1,226 Negative 145.1 ->127.07 94 145.1 ->109.010 94L-Cystine 1,239 Negative 239.0 ->120.04 74 239.0 ->74.120 74trans-4-Hydroxy-L-proline 1,252 Negative 130.1 ->128.012 100 130.1 ->66.016 100D-Xylose 1,259 Negative 149.1 ->89.04 68 149.1 ->71.04 68 ### Page 7 L-Homoserine 1,261 Negative 118.0 ->55.016 70 118.0 ->72.110 70L-Threonine 1,27 Negative 118.0 ->74.19 72myo-Inositol 1,273 Negative 239.1 ->179.00 72 179.0 ->71.012 72Taurine 1,278 Negative 124.0 ->80.024 122D-Mannose 1,29 Negative 179.1 ->89.04 66 179.1 ->59.216 66gamma-Aminobutyric acid 1,296 Negative 102.0 ->84.18 64L-Arabitol 1,309 Negative 151.0 ->89.012 110 151.0 ->71.016 110Xylitol 1,309 Negative 151.1 ->89.08 96 151.1 ->71.08 96L-Citrulline 1,317 Negative 174.1 ->131.08 88 174.1 ->42.248 88Cellobiose 1,322 Negative 341.1 ->161.05 68D-Maltose 1,322 Negative 341.1 ->161.05 72 341.1 ->179.04 72Melibiose 1,322 Negative 341.1 ->179.04 76 341.1 ->89.013 76N-Acetyl D-galactosamine 1,333 Negative 220.1 ->119.00 68Creatine 1,337 Negative 130.1 ->88.18 66 130.1 ->41.236 66Trehalose 1,339 Negative 401.1 ->341.010 98 341.0 ->89.012 98L-Proline 1,346 Negative 114.0 ->68.112 94 114.0 ->45.212 94 ### Page 8 Allantoin 1,351 Negative 157.0 ->97.012 76 157.0 ->42.216 76L-Canavanine 1,351 Negative 175.1 ->74.010 74 175.1 ->118.08 74Cytosine 1,355 Negative 110.0 ->67.18 58 110.0 ->42.212 584-Guanidobutyric acid 1,362 Negative 144.1 ->102.08 74 144.1 ->41.232 74Creatinine 1,363 Negative 112.0 ->41.224 92 112.0 ->68.116 92L-Arginine 1,393 Negative 173.1 ->131.011 90 173.1 ->156.08 90L-Homocysteine 1,422 Negative 134.0 ->116.98 70L-Arabinose 1,437 Negative 149.0 ->59.212 66 149.0 ->89.14 66L-Homocystine 1,441 Negative 267.0 ->115.016 92 267.0 ->132.08 922-Deoxy-D-ribose 1,485 Negative 193.1 ->59.216 64 193.1 ->133.00 64L-Sorbose 1,536 Negative 179.1 ->89.04 66 179.1 ->71.014 66Ribonic acid gamma lactone 1,539 Negative 147.0 ->59.216 88 147.0 ->99.08 88D-Glucosamine 6-phosphate 1,543 Negative 258.0 ->79.048 182 258.0 ->97.016 182Uracil 1,634 Negative 111.0 ->42.214 86O-Phosphorylethanolamine 1,708 Negative 140.0 ->79.014 122 140.0 ->63.038 122Cytidine 1,771 Negative 242.1 ->109.08 104 242.1 ->42.216 104 ### Page 9 L-Methionine 1,775 Negative 148.0 ->47.212 70 148.0 ->100.06 70Hypoxanthine 1,903 Negative 135.0 ->92.017 104 135.0 ->65.128 1042-Deoxycytidine 1,956 Negative 286.1 ->226.04 64 286.1 ->93.020 64Guanine 1,978 Negative 150.0 ->133.012 116 150.0 ->66.036 116Xanthine 2,054 Negative 151.0 ->108.016 104 151.0 ->42.224 104L-Isoleucine 2,099 Negative 130.0 ->45.016 96L-Leucine 2,099 Negative 130.0 ->45.016 96L-Tyrosine 2,213 Negative 180.0 ->119.015 114 180.0 ->93.012 114Uridine 2,22 Negative 243.1 ->200.06 128 243.1 ->110.012 128AICAR 2,312 Negative 257.1 ->125.010 118 257.1 ->42.244 1183-Hydroxy-DL-kynurenine 2,339 Negative 223.1 ->162.010 88 223.1 ->205.94 88Pyridoxal hydrochloride 2,399 Negative 166.1 ->138.012 104 166.1 ->108.018 104Pyridoxine 2,425 Negative 168.1 ->166.09 92 168.1 ->122.117 922-Deoxyuridine 2,615 Negative 227.1 ->184.06 116 227.1 ->42.220 1165-Methoxytryptamine 2,718 Negative 189.1 ->174.012 92 189.1 ->144.028 92Adenine 2,763 Negative 134.0 ->107.018 118 134.0 ->92.120 118 ### Page 10 Thymine 2,764 Negative 125.0 ->42.214 86±-Mevalonolactone 2,894 Negative 189.1 ->59.16 48Inosine 3,151 Negative 267.1 ->135.022 144 267.1 ->108.044 144beta-Nicotinamide mononucleotide 3,401 Negative 333.0 ->251.112 60 333.0 ->135.136 602-Deoxyinosine 3,412 Negative 251.1 ->135.020 124 251.1 ->108.042 124Guanosine 3,72 Negative 282.1 ->150.017 152 282.1 ->132.932 152L-Kynurenine 3,747 Negative 207.1 ->190.04 76 207.1 ->144.020 762-Deoxyguanosine 4,006 Negative 266.1 ->150.012 74 266.1 ->108.036 74DL-2-Aminoadipic acid 4,077 Negative 160.1 ->142.09 96 160.1 ->116.012 96L-Phenylalanine 4,194 Negative 164.1 ->147.09 100 164.1 ->103.015 100Argininosuccinic acid 4,222 Negative 289.0 ->131.127 98 289.0 ->88.036 98L-Glutamic acid 4,287 Negative 146.0 ->102.011 76 146.0 ->128.08 76L-Aspartic Acid 4,488 Negative 132.0 ->88.010 76 132.0 ->71.014 764-Hydroxy-L-glutamic acid 4,68 Negative 162.0 ->144.06 72 162.0 ->72.116 72Glucoheptonic acid 4,986 Negative 225.1 ->129.011 98 225.1 ->75.118 98S-5-Adenosyl-L-homocysteine 4,992 Negative 383.1 ->134.024 130 383.1 ->248.013 130Thymidine 5,027 Negative 241.1 ->42.216 122 241.1 ->151.06 122 ### Page 11 D-Gluconic acid 5,23 Negative 195.1 ->75.218 104 195.1 ->129.010 104Galactonic acid 5,23 Negative 195.1 ->75.118 94 195.1 ->129.011 94Uric acid 5,526 Negative 167.0 ->124.013 128 167.0 ->42.326 128Glyceric acid 5,628 Negative 105.0 ->75.18 70 105.0 ->57.214 70Glyoxylic acid 5,906 Negative 73.0 ->45.26 48N-Acetylneuraminic acid 5,932 Negative 308.1 ->87.014 94 308.1 ->169.910 94Quinic acid 6,063 Negative 191.1 ->85.125 124 191.1 ->93.124 124O-Succinyl-L-homoserine 6,154 Negative 218.1 ->117.07 68 218.1 ->118.00 68Adenosine 6,227 Negative 266.0 ->107.044 122 266.0 ->134.010 72Shikimic acid 6,231 Negative 173.0 ->111.07 132 173.0 ->93.116 1322-Deoxyadenosine 6,478 Negative 310.1 ->250.04 74 250.0 ->134.112 74Oxamic acid 6,502 Negative 88.0 ->44.36 44 88.0 ->42.312 44Chorismic acid 6,691 Negative 225.0 ->189.06 68Succinic semialdehyde 6,964 Negative 101.0 ->57.27 72Lactic acid 7,225 Negative 89.0 ->43.310 48 89.0 ->45.39 483-Dehydroshikimic acid 7,316 Negative 171.0 ->127.08 86 171.0 ->109.017 86alpha-D(+)Mannose 1-phosphate 7,37 Negative 259.0 ->97.014 100 259.0 ->79.048 100 ### Page 12 Creatine phosphate 7,424 Negative 210.0 ->79.016 70 210.0 ->96.94 70L-Dihydroorotic acid 7,502 Negative 157.0 ->113.05 116 157.0 ->42.230 116alpha-D-Glucose-1-phosphate 7,589 Negative 259.0 ->79.028 126 259.0 ->240.99 126D-Glucose 6-phosphate 7,597 Negative 259.0 ->97.014 102 259.0 ->79.048 102L-Tryptophan 7,761 Negative 203.1 ->116.014 116 203.1 ->74.114 116D-Ribose 5-phosphate 7,784 Negative 229.0 ->79.048 96 229.0 ->97.010 96D-Sedoheptulose-7-phosphate 7,891 Negative 289.0 ->96.918 104 289.0 ->79.048 104Trehalose 6-phosphate 7,923 Negative 421.1 ->241.027 176gamma-Glu-Cys 7,935 Negative 249.1 ->128.08 94 249.1 ->171.09 94D-Fructose 6-phosphate 7,942 Negative 259.0 ->97.014 102 259.0 ->79.048 102Xanthosine 8,093 Negative 283.1 ->151.018 126 283.1 ->108.040 126D-Xylulose-5-phosphate 8,322 Negative 229.0 ->79.036 86 229.0 ->139.08 86N-Acetyl-D-glucosamine 6-phosphate 8,672 Negative 300.0 ->97.018 86 300.0 ->79.048 86Nicotinic acid mononucleotide 8,73 Negative 334.0 ->289.94 70 334.0 ->79.040 70Orotic acid 8,921 Negative 155.0 ->42.026 68 155.0 ->40.044 682-Deoxy-D-glucose 6-phosphate 8,927 Negative 243.0 ->79.145 96 243.0 ->97.014 96 ### Page 13 6-Hydroxynicotinic acid 9,048 Negative 138.0 ->94.110 72 138.0 ->42.327 72N-Acetyl-alpha-D-glucosamine 1-phosphate 9,11 Negative 300.0 ->97.016 132 300.0 ->79.036 132D-erythro-Dihydrosphingosine 9,118 Negative 300.3 ->199.08 110 300.3 ->282.06 1104-Quinolinol 9,241 Negative 144.0 ->116.028 160 144.0 ->66.144 1602-Deoxyribose 5-phosphate 9,247 Negative 213.0 ->79.140 92 213.0 ->96.912 92beta-Nicotinamide adenine dinucleotide 9,452 Negative 662.1 ->540.012 70 662.1 ->327.936 702-Methyl-1-butanol 9,468 Negative 87.1 ->43.35 38Pyruvic acid 9,468 Negative 87.0 ->43.24 48Cytidine-5-monophosphate 9,512 Negative 322.0 ->79.044 130 322.0 ->97.022 130o-Phospho-L-Serine 9,911 Negative 184.0 ->79.036 132 184.0 ->97.013 1324-Aminobenzoic acid 9,921 Negative 136.0 ->92.08 722-Deoxycytidine 5-monophosphate 10,235 Negative 306.0 ->194.914 118 306.0 ->79.044 118Glutathione (reduced) 10,235 Negative 306.1 ->195.016 60 306.1 ->110.020 60Dihydroxyacetone phosphate 10,591 Negative 169.0 ->97.06 128 169.0 ->79.032 128Uridine 5-monophosphate 10,594 Negative 323.0 ->79.148 126 323.0 ->97.024 1262-3-Dihydroxyisovalerate 10,618 Negative 133.1 ->75.110 88 133.1 ->57.215 88 ### Page 14 Inosine 5-monophosphate 10,904 Negative 347.0 ->79.044 150 347.0 ->97.022 1505-Deoxy-5-(methylthio)adenosine 11,717 Negative 356.1 ->296.04 70 356.1 ->134.012 70DL-Glyceraldehyde 3-phosphate 11,814 Negative 169.0 ->79.028 130 169.0 ->97.04 130Nicotinic acid 11,816 Negative 122.0 ->78.111 60 122.0 ->51.232 603-Hydroxyanthranilic acid 11,891 Negative 152.0 ->108.012 86 152.0 ->107.023 862-Deoxyguanosine 5-monophosphate 11,97 Negative 346.0 ->79.148 122 346.0 ->97.036 122Adenosine 5-monophosphate 11,97 Negative 346.0 ->79.038 146 346.0 ->97.024 146D-pantothenic acid 11,997 Negative 218.0 ->146.114 70 218.0 ->88.010 704-Hydroxybenzoic acid 12,009 Negative 137.0 ->93.014 88 137.0 ->65.234 88Guanosine 3,5-cyclic monophosphate 12,171 Negative 344.0 ->150.024 104 344.0 ->133.044 104Prephenic acid 12,333 Negative 225.0 ->101.09 842-Ketobutyrate 12,396 Negative 101.0 ->57.24 64Riboflavin 12,523 Negative 375.1 ->255.014 170 375.1 ->212.028 170Vanillic acid 12,586 Negative 167.0 ->123.09 82 167.0 ->108.018 82Maleic acid 12,648 Negative 115.0 ->71.27 62 115.0 ->27.315 62Epicatechin 12,677 Negative 289.1 ->245.011 128 289.1 ->109.026 128 ### Page 15 2-Deoxyadenosine 5-monophosphate 12,693 Negative 330.0 ->79.048 128 330.0 ->134.128 128Succinic acid 12,842 Negative 117.0 ->73.110 62 117.0 ->99.08 62Isopentenyl pyrophosphate 12,913 Negative 245.0 ->209.04 525-Hydroxy-3-indoleacetic acid 12,939 Negative 190.1 ->144.021 74 190.1 ->116.046 74N-Formyl-L-Tyrosine 12,939 Negative 208.1 ->164.07 122 208.1 ->107.012 122Malonic acid 13,002 Negative 103.0 ->59.27 56 103.0 ->41.332 56N-Carbamoyl-DL-aspartic acid 13,378 Negative 175.0 ->132.07 76 175.0 ->88.119 76Adenosine 3-5-cyclic monophosphate 13,385 Negative 328.0 ->134.024 154 328.0 ->79.048 154L-Gluthathione (oxidized) 13,433 Negative 611.1 ->306.124 152 611.1 ->272.128 152N-Carbamyl-L-glutamic acid 13,454 Negative 189.1 ->102.120 78 189.1 ->146.07 78Citramalic acid 13,507 Negative 147.0 ->87.014 76 147.0 ->85.112 76m-Hydroxybenzoic acid 13,508 Negative 137.0 ->93.110 92 137.0 ->65.228 92L-Malic acid 13,601 Negative 133.0 ->115.08 76 133.0 ->71.114 764-Methyl-2-oxovaleric acid 13,677 Negative 129.1 ->85.17 74Itaconic acid 13,677 Negative 129.0 ->85.16 68 129.0 ->41.312 68L-Hydroxyglutaric acid 13,692 Negative 147.0 ->128.97 74 147.0 ->85.113 74 ### Page 16 Mevalonic acid 13,692 Negative 147.1 ->128.98 52 147.1 ->103.111 522-2-Dimethyl Succinic acid 13,802 Negative 145.1 ->101.111 88 145.1 ->127.18 88Adipic acid 13,802 Negative 145.1 ->101.111 78 145.1 ->83.112 78N-acetyl aspartate 13,886 Negative 174.0 ->88.016 60 174.0 ->58.120 60N-Acetylglutamic acid 13,978 Negative 188.1 ->128.010 86 188.1 ->102.016 86Uridine 5'-diphosphogalactose 14,092 Negative 565.0 ->322.924 180 565.0 ->384.929 180Uridine 5-diphosphoglucose 14,092 Negative 565.0 ->322.924 152 565.0 ->384.928 1523-Hydroxyphenylacetic acid 14,139 Negative 151.0 ->107.14 58 151.0 ->65.226 583-Methylglutaric acid 14,182 Negative 145.1 ->101.111 82 145.1 ->41.352 82alpha-Ketoglutaric acid 14,182 Negative 145.0 ->101.05 70 145.0 ->57.29 704-Hydroxyphenyl-pyruvic acid 14,256 Negative 179.0 ->107.04 68Cytidine 5-diphosphate 14,287 Negative 402.0 ->79.048 108 402.0 ->158.924 108Isopentyl acetate 14,293 Negative 129.1 ->85.26 134 129.1 ->41.212 134Ketovaleric acid 14,505 Negative 115.0 ->71.24 703-phosphoglyceric acid 14,552 Negative 185.0 ->79.030 80 185.0 ->97.030 80trans-trans Muconic acid 14,563 Negative 141.0 ->53.38 64 ### Page 17 D-Ribulose 1,5-biphosphate 14,601 Negative 308.9 ->97.022 80 308.9 ->79.046 802-3-Dihydroxybenzoic acid 14,63 Negative 153.0 ->109.015 96 153.0 ->53.225 96Mevalonic acid 5-phosphate 14,66 Negative 227.0 ->79.040 88 227.0 ->97.012 88Uridine 5-diphosphate 14,684 Negative 403.0 ->158.928 108 403.0 ->79.048 108Guanosine 5-diphosphate 14,709 Negative 442.0 ->150.031 144 442.0 ->158.920 1442-Deoxycytidine 5-diphosphate 14,752 Negative 386.0 ->97.020 106 386.0 ->79.036 1062-3-Pyridinedicarboxylic acid 14,798 Negative 166.0 ->122.06 68 166.0 ->78.113 68Pyridoxal 5 phosphate 14,804 Negative 246.0 ->79.030 70D-Fructose 1,6-biphosphate 14,871 Negative 338.9 ->241.012 90 338.9 ->97.022 90trans-Aconitic acid 14,924 Negative 173.0 ->129.04 58 173.0 ->85.110 58Citric acid 15,015 Negative 191.0 ->111.010 78 191.0 ->87.016 78DL-Isocitric acid 15,015 Negative 191.0 ->111.011 74 191.0 ->173.06 742-4-Quinolinediol 15,102 Negative 160.0 ->42.229 120 160.0 ->117.919 120Folinic acid 15,13 Negative 472.2 ->343.020 160Phosphoenolpyruvic acid 15,186 Negative 167.0 ->79.012 118Inosine 5-diphosphate 15,215 Negative 427.0 ->158.928 90 427.0 ->135.024 90 ### Page 18 Homocitrate 15,218 Negative 205.0 ->125.010 96 205.0 ->145.010 962-Deoxyguanosine 5-diphosphate 15,224 Negative 426.0 ->158.928 102 426.0 ->79.148 102Adenosine 5-diphosphate 15,224 Negative 426.0 ->159.028 56 426.0 ->328.016 564-Pyridoxic acid 15,227 Negative 182.0 ->138.012 84 182.0 ->108.120 84cis-Aconitic acid 15,253 Negative 173.0 ->129.04 62 173.0 ->85.18 62Thymidine 5-diphosphate 15,267 Negative 401.0 ->79.044 114 401.0 ->97.025 1143-2-Hydroxyethylindole 15,431 Negative 160.1 ->142.116 90 160.1 ->130.114 902-Isopropylmalic acid 15,464 Negative 175.1 ->113.013 92 175.1 ->115.013 922-Deoxyadenosine 5-diphosphate 15,621 Negative 410.0 ->79.144 158 410.0 ->158.724 158Indoline-2-carboxylate 16,208 Negative 162.1 ->116.016 114 162.1 ->118.011 114Adenylosuccinic acid 16,314 Negative 462.0 ->97.024 148 462.0 ->134.148 148Ketoisovaleric acid 16,487 Negative 115.0 ->71.24 703-Indoleacetic acid 16,528 Negative 174.1 ->130.07 74 174.1 ->128.019 74o-Hydroxy hippuric acid 16,561 Negative 194.0 ->150.011 86 194.0 ->121.022 86Lipoamide 16,721 Negative 204.1 ->158.04 70 204.1 ->64.120 70Salicylic acid 16,74 Negative 137.0 ->93.117 78 137.0 ->65.233 78UDP-Gluceronic Acid 16,751 Negative 579.0 ->323.020 156 579.0 ->158.840 156 ### Page 19 2-Deoxyuridine 5-triphosphate 16,753 Negative 467.0 ->158.836 144 467.0 ->368.920 144Deoxycytidine 5-triphosphate 16,778 Negative 466.0 ->158.928 142 466.0 ->367.920 142Cytidine 5-triphosphate 16,828 Negative 482.0 ->158.840 142 482.0 ->79.040 142Flavin adenine dinucleotide 16,927 Negative 784.1 ->437.030 148 784.1 ->346.035 148Guanosine 5-triphosphate 16,937 Negative 522.3 ->158.936 102 522.3 ->424.020 102PRPP 16,948 Negative 389.0 ->291.012 60 389.0 ->176.720 60Uridine 5-triphosphate 16,988 Negative 483.0 ->158.936 146 483.0 ->385.020 146Inosine 5-triphosphate 17,156 Negative 507.0 ->159.040 124 507.0 ->409.019 124Adenosine 5-triphosphate 17,165 Negative 506.0 ->159.038 122 506.0 ->408.122 122Deoxyguanosine 5-triphosphate 17,165 Negative 506.0 ->158.832 150 506.0 ->407.920 150Deoxyadenosine 5-triphosphate 17,173 Negative 490.0 ->391.924 148 490.0 ->158.932 148Deoxythymidine 5-triphosphate 17,208 Negative 481.0 ->158.836 130 481.0 ->383.120 130Phenylpyruvic acid 17,278 Negative 163.0 ->91.16 68Coenzyme A 17,812 Negative 766.0 ->686.040 135 766.0 ->419.040 135Acetyl-Coenzyme A 17,996 Negative 808.0 ->461.040 135 808.0 ->728.040 135Acetoacetyl-Coenzyme A 18,012 Negative 850.0 ->686.040 135 850.0 ->766.040 135 ### Page 20 3-Hydroxybutyryl-Coenzyme A 18,128 Negative 852.0 ->505.040 135Malonyl-Coenzyme A 18,145 Negative 852.0 ->808.040 135 852.0 ->728.040 135Methylmalonyl-Coenzyme A 18,203 Negative 866.0 ->822.040 135 866.0 ->742.040 135Succinyl-Coenzyme A 18,211 Negative 866.0 ->519.040 135 866.0 ->786.040 135Palmitoyl-Coenzyme A 18,32 Negative 1004.0 ->924.040 135 1004.0 ->657.040 135Proprionyl-Coenzyme A 18,534 Negative 822.0 ->475.040 135 822.0 ->742.040 135Butyryl-Coenzyme A 18,861 Negative 836.0 ->756.040 135 836.0 ->489.040 135Isovaleryl-Coenzyme A 19,002 Negative 850.0 ->770.040 135 850.0 ->503.040 135Taurocholic acid 20,659 Negative 514.0 ->514.050 150Glycocholic acid 20,751 Negative 464.0 ->74.040 215 464.0 ->402.040 215Chenodeoxycholic acid 21,312 Negative 391.0 ->373.036 190Glycodeoxycholic acid 21,427 Negative 448.0 ->74.040 195 448.0 ->386.040 195 ### Page 21 Supplementary Table 2. Reversed Phase and Ion Paired MS parameters for targetedmetabolomics MS, Mass spectrometryReversed Phase MS parametersMass Spectrometer Agilent 6470Ion Source AJS ESIPolarity Value (+)Gas Temp (°C) 200Gas Flow (l/min) 13Nebulizer (psi) 45SheathGasHeater 325SheathGasFlow 12Capillary (V) 3000VCharging 500Ion Paired MS parametersMass Spectrometer Agilent 6470Ion Source AJS ESIPolarity Value (-)Gas Temp (°C) 150Gas Flow (l/min) 13Nebulizer (psi) 45SheathGasHeater 325SheathGasFlow 12Capillary (V) 2000VCharging 500 ### Page 22 Supplementary Table 3. Reversed phase LC parametersLC, liquid chromatographyQuaternary pump G7104AFlow 0.400 mL/minUse Solvent Types YesLow Pressure Limit 0.00 barHigh Pressure Limit 1200.00 barMax. Flow Ramp Up 100.000 mL/min²Primary Channel AutomaticMax. Flow Ramp Down 100.000 mL/min²StrokeAutomatic Stroke Calculation YesMixer SelectionSelected Mixer Do not use MixerBlend AssistEnabled NoStop TimeStoptime Mode Time setStoptime 11.00 minPost TimePosttime Mode OffLC SolventsChannel Solvent Composition UsedA 100% H2O, w/ 0.1% Formic Acid YesB 90:10 Acetonitrile:H2O, 0.1% Formic Acid YesC 100% H2O NoD 100% Isopropanol NoLC gradient programTime (min) A (%) B (%) C (%) D (%) Flow(mL/min)Pressure(bar)2.00 min 100.00 % 0.00 % 0.00 % 0.00 % --- mL/min --- bar7.10 min 1.00 % 99.00 % 0.00 % 0.00 % --- mL/min --- bar8.00 min 1.00 % 99.00 % 0.00 % 0.00 % 0.400mL/min--- bar8.10 min 100.00 % 0.00 % 0.00 % 0.00 % 0.600mL/min--- bar ### Page 23 10.00 min 100.00 % 0.00 % 0.00 % 0.00 % 0.600mL/min--- bar10.10 min 100.00 % 0.00 % 0.00 % 0.00 % 0.400mL/min--- bar11.00 min 100.00 % 0.00 % 0.00 % 0.00 % --- mL/min --- barColumn Compartment G7116BTemperature Control Mode Temperature SetTemperature 50.0 °CEnable Analysis Left TemperatureEnable Analysis Left Temperature On NoRight Temperature ControlRight temperature Control Mode CombinedEnable Analysis Right TemperatureEnable Analysis Right Temperature On NoEnforce column for runEnforce column for run enabled NoStop TimeStoptime Mode As pump/injectorPost TimePosttime Mode OffValve Position Position 2 (Port 1 -> 2)Ready when front door open YesPosition Switch After Run Do not switch ### Page 24 Supplementary Table 4. Ion Paired LC parametersLC, liquid chromatographyQuaternary pump G7104AFlow 0.250 mL/minUse Solvent Types YesLow Pressure Limit 0.00 barHigh Pressure Limit 1200.00 barMax. Flow Ramp Up 100.000 mL/min²Primary Channel AutomaticMax. Flow Ramp Down 100.000 mL/min²StrokeAutomatic Stroke Calculation YesMixer SelectionSelected Mixer Do not use MixerBlend AssistEnabled NoStop TimeStoptime Mode Time setStoptime 40.00 minPost TimePosttime Mode OffLC SolventsChannel Solvent Composition UsedA 97:3 Aq:MeOH w/ 10mM TBA, 15mM AceticAcidYesB 100% Isopropanol NoC 97:3 MeOH:H20 w/ 10mM Tributylamine, 15mMAcetic AcidYesD 99% ACN YesLC gradient programTime (min) A (%) B (%) C (%) D (%) Flow(mL/min)Pressure limit(bar)2.50 min 100.00 % 0.00 % 0.00 % 0.00 % 0.250 mL/min 1200.00 bar7.50 min 80.00 % 0.00 % 20.00 % 0.00 % 0.250 mL/min 1200.00 bar13.00 min 55.00 % 0.00 % 45.00 % 0.00 % 0.250 mL/min 1200.00 bar20.00 min 1.00 % 0.00 % 99.00 % 0.00 % 0.250 mL/min 1200.00 bar ### Page 25 24.00 min 1.00 % 0.00 % 99.00 % 0.00 % 0.250 mL/min 1200.00 bar24.05 min 1.00 % 0.00 % 0.00 % 99.00 % 0.250 mL/min 1200.00 bar27.00 min 1.00 % 0.00 % 0.00 % 99.00 % 0.250 mL/min 1200.00 bar27.50 min --- % --- % 0.00 % 99.00 % 0.800 mL/min 1200.00 bar31.35 min --- % --- % 0.00 % 99.00 % 0.800 mL/min 1200.00 bar31.50 min 1.00 % 0.00 % 0.00 % 99.00 % 0.600 mL/min 1200.00 bar32.25 min 100.00 % 0.00 % 0.00 % 0.00 % 0.400 mL/min 1200.00 bar39.90 min 100.00 % 0.00 % 0.00 % 0.00 % 0.400 mL/min 1200.00 bar40.00 min 100.00 % 0.00 % 0.00 % 0.00 % 0.250 mL/min 1200.00 barColumn Compartment G7116BTemperature Control Mode Temperature SetTemperature 35.0 °CEnable Analysis Left TemperatureEnable Analysis Left Temperature On YesEnable Analysis Left Temperature Value 0.8 °CLeft Temp. Equilibration Time 0.0 minRight Temperature ControlRight temperature Control Mode CombinedEnable Analysis Right TemperatureEnable Analysis Right Temperature On YesEnable Analysis Right Temperature Value 0.8 °CRight Temp. Equilibration Time 0.0 minEnforce column for runEnforce column for run enabled NoStop TimeStoptime Mode As pump/injectorPost TimePosttime Mode OffValve Position Position 2 (Port 1 -> 2)Ready when front door open YesPosition Switch After Run Do not switchTime (min) Function Parameter24.00 min Change Valve Position Column at Position 1 ### Page 26 39.00 min Change Valve Position Column at Position 2 ### Page 27 Supplementary Table 5: Metabolite trajectories of CNS-associated metabolites duringneuro-inflammationTable represents membership degree of the specified metabolites to their assigned cluster. Ahigh membership degree represents a strong affiliation to the assigned cluster. SC, SpinalCord; CSF, cerebrospinal fluid.SC Cluster 1 CSF Cluster 1Metabolite MembershipDegreeMetabolite MembershipDegreeCitrate 0,7590 Isovalerylcarnitine 0,7179Methylbutyrylcarnitine 0,7586 Tryptophan 0,7099Isovalerylcarnitine 0,7574 Uric Acid 0,7070Isobutyrylcarnitine 0,7542 Quinolinic Acid 0,7062N-Acetylgalactosamine 0,7440 Pyruvate 0,7053Nudifloramide 0,7439 Pyridoxal 0,6996Butyrylcarnitine 0,7427 Allantoin 0,6962CTP 0,7411 Riboflavin 0,6793Xanthosine 0,7386 Methylbutyrylcarnitine 0,6766Oleoylcarnitine 0,7365 Histidine 0,6598Hexanoylcarnitine 0,7358 Orotic Acid 0,6521UricAcid 0,7243 O-Phosphorylethanolamine 0,6185Octanoylcarnitine 0,7190 Citrulline 0,6092Thymidine 0,7165 N-Carbamoyl-aspartic acid 0,6087L-arnitine 0,7159 Pantothenic Acid 0,6057O-Acetylcarnitine 0,7128 alpha-Ketoglutaric Acid 0,5966N-alpha-acetylglutamine 0,7058 Hydroxyindoleacetate 0,5930ATP 0,7044 Threonine 0,5650alpha-N-Acetyl-Lysine 0,6982 Homocitrate 0,5641trans-4-Hydroxy-proline 0,6851 Methylnicotinamide 0,5563Taurine 0,6691 N-Methyl-4-Pyridone-3-Carboxamide 0,5498Quinolinic Acid 0,6615 Nicotinic Acid 0,5299GTP 0,6546 Mannose-1-phosphate 0,5132CoA 0,6513 Cytidine 0,5121Glutarylcarnitine 0,6500 Isobutyrylcarnitine 0,5096Itaconic Acid 0,6353 Argininosuccinate 0,4977Dimethyltryptamine 0,6306 Glutarylcarnitine 0,4957Malate 0,6305 Indoxyl-3-sulfate 0,4903Deoxyuridine 0,6283 Malate 0,4861UDP-Glucuronate 0,6266 Fructose-6-phosphate 0,4855Dihydrosphingosine 0,6243 Methylcitric Acid 0,4810Anthranillic Acid 0,6212 Butyrylcarnitine 0,4806Fumarate 0,6030 Fumarate 0,4773CDP 0,6006 Itaconic Acid 0,4629Decanoylcarnitine 0,5920 Thymidine 0,4579 ### Page 28 Tyrosine 0,5858 Xanthosine 0,4502O-Phosphorylethanolamine 0,5844Propionylcarnitine 0,5820Palmitoylcarnitine 0,5779alpha-Ketoglutaric Acid 0,5647UDP-N-acetylgalactosglucosamine0,5519Guanidinobutyric Acid 0,5313ITP 0,5217Pantothenic Acid 0,5187Tryptophan 0,5136Allantoin 0,5088Lauroylcarnitine 0,4956UTP 0,4947IMP 0,4926UDP 0,4603N-Acetyl-glucosamine-6phosphate0,4508Isoleucine 0,4416Methylnicotinamide 0,4360Indoxyl-3-sulfate 0,4355UDP-glucose 0,4309UDP-galactose 0,4301N-Methyl-4-Pyridone-3Carboxamide0,4128Pyridoxic Acid 0,3846Acetyl-CoA 0,3811cAMP 0,3765Nicotinamide Mononucleotide 0,3761Glutathione Oxidized 0,3754FAD 0,3630Pyridoxal 0,3533ShikimicAcid 0,3451Serine 0,4480Phenylalanine 0,4478Lactate 0,4465Propionylcarnitine 0,4390UDP-Glucuronate 0,4385UDP-N-acetylgalactosglucosamine 0,4290Glutamine 0,4246Asparagine 0,4151Isoleucine 0,4073Kynurenine 0,4069N-alpha-acetyl-glutamine 0,4051N-Acetylglutamate 0,4033Methionine 0,3955Citrate 0,3728Quinic Acid 0,3657CSF Cluster 2Metabolite MembershipDegreeDihydroxyacetone Phosphate 0,6241Adenosine 0,6222Creatine 0,6124Hyroxykynurenine 0,6089Methylthioadenosine 0,5933N-Acetyl-Glycine 0,5898Adenine 0,5788Methylmalonate 0,5750Glutathione Oxidized 0,5629 ### Page 29 SC Cluster 2Metabolite MembershipDegreeHistidine 0,6821S-Adenosyl-Methionine 0,6804Methylthioadenosine 0,6804Ophthalmate 0,6788dCMP 0,6693Methylcitric Acid 0,6682Methionine 0,6527Aminoadipic Acid 0,6526alpha-Glucose-1-phosphate 0,6504alpha-N-Acetyl-L-Arginine 0,6462Fructose-1-6-biphosphate 0,6430Creatinine 0,5555UTP 0,5400N-Acetyl-valine 0,5357N-Carbamyl_-glutamic acid 0,5118UMP 0,5025NAD 0,4999Taurine 0,4854IMP 0,4778Glutathione Reduced 0,4728Glutamate 0,4650D-Maltose 0,4577Leucine 0,4573O-Acetylcarnitine 0,4486L-arnitine 0,4408alpha-Ribose-5-phosphate 0,4403S-Adenosyl-Homocysteine 0,4372Uracil 0,6404Cytidine 0,6392N-Acetyl-L-Glycine 0,6307Cystathionine 0,6211Pyruvate 0,6125Mannose-1-phosphate 0,6028UMP 0,5993N-Acetylglutamate 0,5733dTTP 0,5678dAMP 0,5604D-Sedoheptulose-7phosphate 0,5489Serine 0,5477PRPP 0,5388Phenylalanine 0,5387AMP 0,4369Gluconate 0,4266Anthranillic acid 0,4245Inosine 0,4189UDP-glucose 0,4106cAMP 0,3997Uracil 0,3981Phosphoglycerate 0,3976Xanthine 0,3927dTDP 0,3834N-Acetyl-Aspartate 0,3735Tyrosine 0,3654Nicotinamide mononucleotide 0,3645 ### Page 30 Hydroxyglutaric acid 0,5366Glucose-6-phosphate 0,5285CMP 0,5077Methylmalonic Acid 0,5074Fructose-6-phosphate 0,5065Pyridoxal-5-phosphate 0,4969Xanthine 0,4845N-Acetyl-alpha-glucosamine1-phosphate0,4835Succinate 0,4688Kynurenine 0,4687Phosphoglycerate 0,4641Threonine 0,4593O-Phospho-Serine 0,4586Argininosuccinate 0,4488Orotic Acid 0,4456Citrulline 0,4446Ophthalmic Acid 0,4358Homocitrate 0,4303Hydroxyindoleacetate 0,4301Dihydroxyacetone Phosphate 0,3864Deoxy-glucose-6-phosphate 0,3505CSF Cluster 3Metabolite MembershipDegreeXylose 0,6574L-Arabitol-Xylitol 0,6526Mannose 0,6486N-Acetylneuraminic Acid 0,6480Arginine 0,6412Sorbose 0,6401Shikimic Acid 0,6148S-Adenosyl-Methionine 0,6048Arabinose 0,5588myo-Inositol 0,5263Glyceric Acid 0,5193Guanosine 0,4641Cystine 0,4537N-Acetylglucosamine 0,4508Nicotinamide-N-oxide 0,4135Hypoxanthine 0,3936Nudifloramide 0,3920Thiamine 0,3876Uridine 0,3863Pyridoxal-5-phosphate 0,3785trans-4-Hydroxy-proline 0,3679Deoxyuridine 0,3577SC Cluster 3Metabolite MembershipDegreeCreatine 0,7599N-Acetyl-Aspartate 0,7575N-Carbamoyl-aspartic acid 0,7496dTDP 0,7455ADP 0,7423S-Adenosyl-Homocysteine 0,7083L-Arabitol-Xylitol 0,6838Adenosine 0,6754Mannose 0,6740Sorbose 0,6550Guanosine 0,6522AMP 0,6520GDP 0,6420Adenine 0,6224Asparagine 0,6198myo-Inositol 0,6144Lactate 0,6007Inosine 0,5864 ### Page 31 Glutamate 0,5725alpha-Ribose-5-phosphate 0,5589Creatinine 0,5539N-Acetylneuraminic Acid 0,5385Arginine 0,5317Leucine 0,5314Glutathione Reduced 0,5313Nicotinamide 0,5296NicotinicAcid 0,5285N-Carbamyl-glutamic acid 0,5143NAD 0,5094Guanine 0,5047Uridine 0,4992GDP-Mannose 0,4963Xylulose-5-phosphate 0,4938Trehalose 0,4793gamma-Glu-Cys 0,4633Methylmalonate 0,4569Hypoxanthine 0,4458Thiamine 0,4339Adenylosuccinate 0,4299IDP 0,4120Maltose 0,3925N-Acetylglucosamine 0,3687Glutamine 0,3590N-methyl-Nicotinamide 0,3528 ### Page 32 Supplementary Table 6. m/z and RT of derivatized polyaminesCompound Derivatized m/z Retention TimeISTD-D8Tryptophan 213.1474 2.5N1-N8-diacetylspermidine 330.2387 3.2N-acetylputrescine 231.1703 3.3Arginine 375.2238 3.4Gamma-Aminobutyrate 104.0706 3.4N-acetylcadaverine 245.186 3.5N1N12 diacetylspermine 487.349 4.3Ornithine 333.202 4.4N8-Acetylspermidine 388.2806 4.8N1-Acetylspermidine 388.2806 4.81,3 Diaminopropane 275.1965 5.1Putrescine 289.2122 5.3Cadaverine 303.2278 5.7ISTD-16Diaminohexane 317.2435 6.2N1-Acetylspermine 545.3909 6.4N-(3-oxopropyl) acetamide 316.1755 7Spermidine 446.3225 7.1Spermine 603.4327 8.4 ### Page 33 Supplementary Table 7. MS patient characteristics and controls used in Fig.3RRMS, relapsing remitting multiple sclerosis; SPMS, secondary progressive multiple sclerosis; AMS, acute MS; PPMS, primary progressivemultiple sclerosisCase ID patient ID Sex ClinicalCourseage DiseaseDuration(month)LesionLocalizationpreDMTera LesionType18-78 1 m RRMS 65 25x12 supratentorial yes inactive328-78 2 m SPMS 34 2x12 supratentorial yes active434-79 3 f PPMS 30 7x12 supratentorial yes chronic active241-84 4 f RRMS 46 18x12 supratentorial yes inactive73-85 5 m AMS 28 6 supratentorial yes active215-86 6 f RRMS 78 26x12 supratentorial yes inactive251-87 7 f RRMS 59 37x12 supratentorial yes chronic active7-02 8 f AMS 37 7 infratentorial no active116-04 9 f AMS 40 6 supratentorial no active19-05 10 f unknown 44 n.a. infratentorial no active75-09 11 f unknown 76 n.a. supratentorial no inactive187-09 12 f PPMS 46 n.a. supratentorial no chronic active217-10 13 f PMS 69 n.a. infratentorial no inactive78-11 14 f unknown 56 n.a. supratentorial no chronic active82-15 15 f SPMS 61 n.a. infratentorial no chronic active101-15 16 m RRMS 52 24x12 supratentorial no chronic active35-16 17 f SPMS 50 >10x12 supratentorial no chronic active194-16 18 m SPMS 73 n.a. supratentorial no inactive ### Page 34 62-04 19 m Control 51 - supratentorial - -79-04 20 m Control 58 - supratentorial - -175-04 21 m Control 77 - supratentorial - -53-06 22 f Control 37 - supratentorial - -195-07 23 f Control 62 - supratentorial - -160-08 24 f Control 86 - supratentorial - -150-09 25 m Control 72 - supratentorial - - ### Page 35 Supplementary Table 8. Statistical matrix for comparisons of populations between different treatment conditions depicted in Ext Fig. 8b24 hours 48 hoursArg1-iNOS- US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSF Arg1-iNOS- US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSFUS <0.0001 <0.0001 <0.0001 <0.0001 US <0.0001 <0.0001 <0.0001 <0.0001IL4/IL13 <0.0001 <0.0001 <0.0001 IL4/IL13 <0.0001 ns <0.0001LPS/IFNg <0.0001 <0.0001 LPS/IFNg <0.0001 0.001LPS/GMCSF <0.0001 LPS/GMCSF <0.0001LPS/IFNg/GMCSFLPS/IFNg/GMCSFArg1-iNOS+ US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSF Arg1-iNOS+ US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSFUS 0.0002 <0.0001 <0.0001 <0.0001 US ns <0.0001 <0.0001 <0.0001IL4/IL13 <0.0001 <0.0001 <0.0001 IL4/IL13 <0.0001 <0.0001 <0.0001LPS/IFNg <0.0001 <0.0001 LPS/IFNg <0.0001 <0.0001LPS/GMCSF <0.0001 LPS/GMCSF <0.0001LPS/IFNg/GMCSFLPS/IFNg/GMCSFArg1+iNOS- US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSF Arg1+iNOS- US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSFUS 0.0002 ns <0.0001 ns US 0.0002 ns <0.0001 ns ### Page 36 IL4/IL13 <0.0001 <0.0001 <0.0001 IL4/IL13 <0.0001 <0.0001 <0.0001LPS/IFNg <0.0001 ns LPS/IFNg <0.0001 nsLPS/GMCSF <0.0001 LPS/GMCSF <0.0001LPS/IFNg/GMCSFLPS/IFNg/GMCSFArg1+iNOS+ US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSF Arg1+iNOS+ US IL4/IL13LPS/IFNgLPS/GMCSFLPS/IFNg/GMCSFUS 0.0119 ns <0.0001 ns US ns <0.0001 <0.0001 <0.0001IL4/IL13 ns <0.0001 <0.0001 IL4/IL13 <0.0001 <0.0001 <0.0001LPS/IFNg <0.0001 <0.0001 LPS/IFNg <0.0001 <0.0001LPS/GMCSF <0.0001 LPS/GMCSF nsLPS/IFNg/GMCSFLPS/IFNg/GMCSF