2f:I[703058,["/_next/static/immutable/chunks/3bhq_5eh1d760.js","/_next/static/immutable/chunks/1855-ogyi8eh4.js","/_next/static/immutable/chunks/2pfsjpn6k6zlg.js","/_next/static/immutable/chunks/26c7v3_3uwnwu.js","/_next/static/immutable/chunks/25a3ozcrvi1ff.js","/_next/static/immutable/chunks/2r4wx50nvjh_4.js","/_next/static/immutable/chunks/23fsw06hsi5b1.js","/_next/static/immutable/chunks/06_yt270h41rg.js","/_next/static/immutable/chunks/3ezsiz-7shkna.js","/_next/static/immutable/chunks/20x2y96ldu5wx.js","/_next/static/immutable/chunks/2lxntcm3pd1_h.js","/_next/static/immutable/chunks/00dgco8irq3jw.js","/_next/static/immutable/chunks/00nwe-kq0rboc.js","/_next/static/immutable/chunks/2051ffx3xzio9.js"],"PaperListPageClient"] 30:T87e,Weaning involves rapid shifts in the gut microbiota, bowel irregularities, and increased infection risks. This exploratory, hypothesis-generating study examined whether daily supplementation with Bifidobacterium longum subsp. infantis M-63 (M-63) may influence bowel function, the gut environment, and general health during weaning. In a randomized, double-blind, placebo-controlled trial, 100 healthy infants and toddlers (5 months to under 3 years) received M-63 (5 billion CFU) or placebo for eight weeks. Stool habits, fecal microbiota, and short-chain fatty acids (SCFAs) were assessed. Cold-like symptoms and medication use were recorded. Exploratory subgroup analyses considered breastfeeding status, age (<1.5 years), and the presence of siblings. M-63 supplementation was associated with more days of normal stools and a trend toward fewer diarrhea episodes. Subgroup analyses revealed that breastfed children showed increased B. infantis abundance. In contrast, non-breastfed children exhibited higher fecal SCFA levels. Children <1.5 years and those with siblings showed trends toward fewer cold-like symptoms and reduced antibiotic use. No intervention-related adverse events occurred. This exploratory trial suggests M-63 may be associated with modest improvements in gastrointestinal outcomes and selected systemic health-related measures during weaning. These findings should be interpreted cautiously and require confirmation in adequately powered trials. Daily administration of B. infantis M-63 was associated with potential improvements in bowel habits among infants. In breastfed children, supplementation with B. infantis M-63 was associated with an observed increase in the relative abundance of B. infantis, alongside a trend toward greater bifidobacterial diversity. In non-breastfed children, B. infantis M-63 was associated with modestly elevated levels of fecal metabolites, including short-chain fatty acids. Exploratory findings suggest that supplementation with B. infantis M-63 during weaning may modulate the gut environment and may be associated with immune-related measures, but confirmation in larger, rigorously designed trials is needed.31:T11a8,Abstract Background Weaning involves rapid shifts in the gut microbiota, bowel irregularities, and increased infection risks. This exploratory, hypothesis-generating study examined whether daily supplementation with Bifidobacterium longum subsp. infantis M-63 (M-63) may influence bowel function, the gut environment, and general health during weaning. Methods In a randomized, double-blind, placebo-controlled trial, 100 healthy infants and toddlers (5 months to under 3 years) received M-63 (5 billion CFU) or placebo for eight weeks. Stool habits, fecal microbiota, and short-chain fatty acids (SCFAs) were assessed. Cold-like symptoms and medication use were recorded. Exploratory subgroup analyses considered breastfeeding status, age (<1.5 years), and the presence of siblings. Results M-63 supplementation was associated with more days of normal stools and a trend toward fewer diarrhea episodes. Subgroup analyses revealed that breastfed children showed increased B. infantis abundance. In contrast, non-breastfed children exhibited higher fecal SCFA levels. Children <1.5 years and those with siblings showed trends toward fewer cold-like symptoms and reduced antibiotic use. No intervention-related adverse events occurred. Conclusions This exploratory trial suggests M-63 may be associated with modest improvements in gastrointestinal outcomes and selected systemic health-related measures during weaning. These findings should be interpreted cautiously and require confirmation in adequately powered trials. Impact

Daily administration of B. infantis M-63 was associated with potential improvements in bowel habits among infants.

In breastfed children, supplementation with B. infantis M-63 was associated with an observed increase in the relative abundance of B. infantis , alongside a trend toward greater bifidobacterial diversity.

In non-breastfed children, B. infantis M-63 was associated with modestly elevated levels of fecal metabolites, including short-chain fatty acids.

Exploratory findings suggest that supplementation with B. infantis M-63 during weaning may modulate the gut environment and may be associated with immune-related measures, but confirmation in larger, rigorously designed trials is needed.

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