2f:I[703058,["/_next/static/immutable/chunks/3bhq_5eh1d760.js","/_next/static/immutable/chunks/1855-ogyi8eh4.js","/_next/static/immutable/chunks/2pfsjpn6k6zlg.js","/_next/static/immutable/chunks/26c7v3_3uwnwu.js","/_next/static/immutable/chunks/25a3ozcrvi1ff.js","/_next/static/immutable/chunks/2r4wx50nvjh_4.js","/_next/static/immutable/chunks/23fsw06hsi5b1.js","/_next/static/immutable/chunks/06_yt270h41rg.js","/_next/static/immutable/chunks/3ezsiz-7shkna.js","/_next/static/immutable/chunks/20x2y96ldu5wx.js","/_next/static/immutable/chunks/2lxntcm3pd1_h.js","/_next/static/immutable/chunks/00dgco8irq3jw.js","/_next/static/immutable/chunks/00nwe-kq0rboc.js","/_next/static/immutable/chunks/2051ffx3xzio9.js"],"PaperListPageClient"] 30:T41e,Highlights across studies: - **Atopic march ML model:** Simplified models using routine early-life clinical features accurately predicted moderate-to-severe persistent asthma/allergic rhinitis by ages 5–11. - **Peanut OIT (IMPACT follow-up):** 80% of children who received pnOIT only (group A, n=40) were eating peanut at follow-up; 35% of group A reported peanut reactions since IMPACT; IgG4:IgE ratio remained higher in the pnOIT group vs. placebo years later. - **Dupilumab & autoimmunity in children:** Cutaneous autoimmune diagnoses (mainly psoriasis and alopecia areata) were ~2 per 1,000 children/year higher in the dupilumab group; no significant differences in endocrine, GI, or rheumatologic autoimmune diagnoses. - **EoE transcriptome:** Dupilumab normalized >1,300 dysregulated EoE genes (eosinophil and non-eosinophil pathways) across all age groups, sustained on treatment. - **α-Gal IgG & atherosclerosis:** Anti-α-Gal IgG formed immune complexes with α-Gal–bearing LDL, enhancing macrophage uptake and foam cell formation in vitro.31:T75a,Major peanut allergens are stable and resistant to denaturation under standard cooking conditions, contributing to allergenicity and low rates of developing natural tolerance in allergic individuals. We evaluated the effects of heat and pressure autoclaving on peanut proteins, IgE binding, and oral tolerability. Raw, roasted, and autoclaved peanut protein extracts were evaluated by Bradford assay, ELISA, and mass spectrometry-proteomics to compare relative amounts of protein, specific IgE binding, and allergen fragmentation. To assess changes in clinical reactivity, we performed skin prick testing (SPT) in 41 subjects using standard and autoclaved peanut extracts. We also performed double-blind oral food challenges (OFC) in 10 peanut-allergic subjects with standard and autoclaved peanuts. Autoclaving at 130°C, 2.4 atm, for 30 min significantly degraded allergens Ara h 1 and 2, and completely degraded Ara h 8. Mass spectrometry-proteomics analysis of size-filtered extracts (< 10 kDa) showed greater numbers and diversity of peptides from peanut proteins and allergens in autoclaved extracts. Autoclaving fragmented proteins into shorter peptides, against which sera from highly allergic patients exhibited a 74% reduction in IgE binding compared to raw peanuts. SPT demonstrated significant decreases in wheal diameters using autoclaved peanut extract (median [IQR] = 5 mm [2, 9]) compared to commercial extract (10 mm [6, 15]; p < 0.001). All OFC subjects tolerated the maximum cumulative autoclaved peanut dose (444 mg) versus standard peanut (median: 9 mg, range: [1, 44]). Autoclaving peanuts induces important chemical changes including fragmentation, leading to decreased peanut allergenicity and consequently increased tolerability. This has the potential for novel immunotherapeutic approaches with more favorable side effect profiles.32:Ta5b,ABSTRACT Background Major peanut allergens are stable and resistant to denaturation under standard cooking conditions, contributing to allergenicity and low rates of developing natural tolerance in allergic individuals. We evaluated the effects of heat and pressure autoclaving on peanut proteins, IgE binding, and oral tolerability. Methods Raw, roasted, and autoclaved peanut protein extracts were evaluated by Bradford assay, ELISA, and mass spectrometry‐proteomics to compare relative amounts of protein, specific IgE binding, and allergen fragmentation. To assess changes in clinical reactivity, we performed skin prick testing (SPT) in 41 subjects using standard and autoclaved peanut extracts. We also performed double‐blind oral food challenges (OFC) in 10 peanut‐allergic subjects with standard and autoclaved peanuts. Results Autoclaving at 130°C, 2.4 atm, for 30 min significantly degraded allergens Ara h 1 and 2, and completely degraded Ara h 8. Mass spectrometry‐proteomics analysis of size‐filtered extracts (< 10 kDa) showed greater numbers and diversity of peptides from peanut proteins and allergens in autoclaved extracts. Autoclaving fragmented proteins into shorter peptides, against which sera from highly allergic patients exhibited a 74% reduction in IgE binding compared to raw peanuts. SPT demonstrated significant decreases in wheal diameters using autoclaved peanut extract (median [IQR] = 5 mm [2, 9]) compared to commercial extract (10 mm [6, 15]; p < 0.001). All OFC subjects tolerated the maximum cumulative autoclaved peanut dose (444 mg) versus standard peanut (median: 9 mg, range: [1, 44]). Conclusions Autoclaving peanuts induces important chemical changes including fragmentation, leading to decreased peanut allergenicity and consequently increased tolerability. This has the potential for novel immunotherapeutic approaches with more favorable side effect profiles. 33:T744,Oral immunotherapies for food allergies remain limited by safety concerns and inconsistent efficacy. We developed a probiotic-based strategy using Escherichia coli Nissle 1917 (EcN) engineered to codisplay the major peanut allergen Ara h 2 and the inhibitory sialic acid-binding immunoglobulin-like lectin (Siglec) ligand Sia(α2-6)Gal(β1-4)Glc to dampen allergen-specific B-cell responses. Expression of Siglec ligand and Ara h 2 on EcN was confirmed by Western blot and flow cytometry. C3H/HeJ mice were sensitized by intraperitoneal injections and treated orally with bacteria. B-cell responses were assessed by flow cytometry after in vitro stimulation of splenocytes. Serum antibody and mast cell protease levels were measured by ELISA. Anaphylaxis protection was evaluated through hypothermia and symptom scores. In vitro stimulation with peanut extract increased B-cell activation in sensitized splenocytes, and wild-type EcN further enhanced this activation. In contrast, EcN displaying Ara h 2 and Siglec ligands significantly reduced CD23 upregulation and restored CD86 and CD69 expression. Oral administration of EcN displaying Ara h 2 and Siglec ligands to peanut-sensitized mice reduced allergen-specific B-cell activation, decreased serum IgE and IgG levels, and significantly attenuated anaphylactic responses. EcN codisplaying Ara h 2 and Siglec ligand conferred protection, highlighting the requirement for antigen-specific engagement of inhibitory B-cell pathways. While this study focused on Ara h 2, including multiple allergens could extend protection. Our findings establish proof of concept for orally delivered antigen-specific immune tolerance using engineered probiotics, providing a scalable, noninvasive alternative to intravenous nanoparticle therapies. This approach could be extended to other allergies or autoimmune conditions.25:[[["$","script","0",{"type":"application/ld+json","dangerouslySetInnerHTML":{"__html":"{\"@context\":\"https://schema.org\",\"@type\":\"CollectionPage\",\"name\":\"Peanut Allergy: condition Research & Clinical Takeaways\",\"description\":\"Explore peer-reviewed papers on Peanut Allergy (condition). Clinician-written summaries and actionable practice insights. 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Please provide the full text or abstract of the article for accurate extraction.","value_numeric":null},{"paper_doi":"10.1016/j.jaci.2026.06.010","tag_type":"warning","value":"The paper provided contains only metadata (DOI, URL, journal, and publication date) with no abstract, body text, or results. No clinical content could be extracted. Please provide the full text or abstract to generate meaningful summaries and tags.","value_numeric":null},{"paper_doi":"10.1016/j.jaci.2026.06.010","tag_type":"warning","value":"The paper provided contains only metadata (title, DOI, journal, and publication date) with no abstract, methods, results, or discussion. No structured summary, tags, or evidence-based content can be extracted. Please provide the full paper text to enable meaningful analysis.","value_numeric":null},{"paper_doi":"10.1016/j.jaci.2026.06.010","tag_type":"warning","value":"The provided source text contains only metadata (DOI, URL, journal name, and publication date) with no abstract, body text, methods, results, or conclusions. Structured summaries and tags cannot be extracted without substantive content. The paper is titled 'The Editors' Choice,' which is typically an editorial or curated highlight piece — its specific focus, population, intervention, and results are entirely unknown from the information provided.","value_numeric":null},{"paper_doi":"10.1016/j.jaci.2026.06.010","tag_type":"warning","value":"The provided source text contains only metadata (DOI, URL, journal name, and publication date) with no abstract, body text, or results. All summary fields and tags have been left null or empty because no content can be extracted or inferred without speculating. Please provide the full paper text for a complete structured extraction.","value_numeric":null},{"paper_doi":"10.1016/j.jaci.2026.06.010","tag_type":"warning","value":"The title 'The Editors' Choice' is a generic editorial section label, not a specific study title. It is unclear which article(s) or topic(s) are being referenced.","value_numeric":null},{"paper_doi":"10.1016/j.jaci.2026.06.010","tag_type":"warning","value":"The α-Gal/foam cell findings are in vitro only; clinical cardiovascular implications should not be overstated.","value_numeric":null},{"paper_doi":"10.1016/j.jaci.2026.06.010","tag_type":"warning","value":"This is an Editors' Choice summary article covering six distinct original studies, not a single primary study — tags and sample sizes reflect the most prominent individual studies cited, not a single pooled population.","value_numeric":null}],"summaries":{"what_studied":"This Editors' Choice issue highlights six original studies covering: (1) ML-based prediction of the atopic march in >10,000 children with early-onset atopic dermatitis; (2) CD25 as a marker of activated MCTCs in chronic rhinosinusitis with nasal polyps; (3) long-term follow-up (~8–11 years) of the IMPACT peanut oral immunotherapy trial in children aged 1–3 years; (4) rates of new autoimmune diagnoses in children ages 6–17 with atopic disease on vs. off dupilumab (PEDSnet, 10 sites, 4-year follow-up); (5) dupilumab's effect on the EoE transcriptome across pediatric, adolescent, and adult patients; and (6) anti-α-Gal IgG antibodies as a mechanistic link between α-Gal sensitization and atherosclerosis.","key_result":"$30","limitations":"- The atopic march ML study and dupilumab/autoimmunity study are both EHR-based and observational, limiting causal inference and subject to coding/ascertainment bias.\n- The IMPACT long-term follow-up enrolled only 78 of 146 original participants (54%), introducing potential selection bias.\n- The α-Gal/atherosclerosis mechanism was demonstrated in vitro only; in vivo cardiovascular relevance remains unproven.","practice_takeaway":"Early-life clinical features (respiratory symptoms, allergy meds, sensitization) can be used to flag young children with eczema for closer monitoring before asthma develops. Children on dupilumab should continue standard longitudinal monitoring for cutaneous autoimmune conditions such as psoriasis and alopecia areata, while being reassured that systemic autoimmune risks appear low."}},{"doi":"10.1001/jamapediatrics.2026.2910","pmid":"42507431","title":"Treatment of Multifood Allergy With Omalizumab or Multiallergen Oral Immunotherapy","journal_title":"JAMA Pediatrics","journal_issn":"2168-6203","published_at":"2026-07-27T00:00:00.000Z","url":"https://doi.org/10.1001/jamapediatrics.2026.2910","source_score":57,"is_retracted":false,"is_open_access":true,"raw_metadata":{"pubmed":true,"crossref":true,"unpaywall":true},"created_at":"2026-07-31T13:36:49.537Z","updated_at":"2026-07-31T13:36:49.537Z","tags":[{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"age_group","value":"mixed","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"brand","value":"Xolair","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"category","value":"Pediatrics","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"condition","value":"Multifood Allergy","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"condition","value":"Peanut Allergy","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"generic","value":"omalizumab","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"impact_flag","value":"practice_changing","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"level_of_evidence","value":"1","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"modality","value":"Anti-IgE Monoclonal Antibody","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"modality","value":"Oral Immunotherapy","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"sample_size","value":"n=117","value_numeric":117},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"sex","value":"mixed","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"study_design","value":"Randomized Controlled Trial","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"warning","value":"Age range (1–55 years) is wide; median age of 7 years means the population is predominantly pediatric, which may limit applicability to adult practice.","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"warning","value":"Brand name 'Xolair' assigned based on standard omalizumab branding; paper does not explicitly state the brand used.","value_numeric":null},{"paper_doi":"10.1001/jamapediatrics.2026.2910","tag_type":"warning","value":"The ITT superiority finding is heavily confounded by differential dropout: 49% of MOIT participants vs. 12% of omalizumab participants discontinued, mostly due to adverse events — per-protocol analysis was not significant.","value_numeric":null}],"summaries":{"what_studied":"This double-blind, placebo-controlled RCT (OUTMATCH stage 2) compared omalizumab monotherapy vs. omalizumab-facilitated multiallergen OIT (MOIT) in 117 participants aged 1–55 years with allergy to peanuts plus ≥2 other foods, across 10 US academic centers over 60 weeks.","key_result":"In the ITT analysis, omalizumab was superior to MOIT for tolerating a cumulative dose ≥4044 mg across all 3 foods (36% vs. 19%; OR 2.6; 95% CI 1.1–6.3; P = .03); however, per-protocol analyses showed no difference. MOIT had far more serious adverse events (31% vs. 0%), discontinuations (22% vs. 0%), and epinephrine use (37% vs. 7%).","limitations":"- The ITT superiority of omalizumab was largely driven by high MOIT discontinuation due to adverse events (49% did not complete), not by greater efficacy in those who tolerated the regimen.\n- Per-protocol analysis showed no significant difference, raising questions about true comparative efficacy.\n- Median participant age was 7 years, limiting direct generalizability to adults.","practice_takeaway":"For children and adults with multifood allergy, omalizumab monotherapy offers a safer profile than multiallergen OIT, with fewer serious reactions and near-zero discontinuations — making it a practical first-line option, especially for patients at high risk of OIT-related reactions. Clinicians should counsel that MOIT's high adverse event burden (31% serious, 22% discontinuation) substantially limits its real-world use in this population."}},{"doi":"10.1111/all.70400","pmid":"42481405","title":"Three‐Year Post‐Treatment Outcomes Following Cessation of Peanut Oral Immunotherapy: Comparisons by Clinical Allergy Status and Treatment Group","journal_title":"Allergy","journal_issn":"0105-4538","published_at":"2026-07-21T00:00:00.000Z","url":"https://doi.org/10.1111/all.70400","source_score":2,"is_retracted":false,"is_open_access":true,"raw_metadata":{"pubmed":true,"crossref":true,"unpaywall":true},"created_at":"2026-08-04T18:18:03.701Z","updated_at":"2026-08-04T18:18:03.701Z","tags":[{"paper_doi":"10.1111/all.70400","tag_type":"age_group","value":"mixed","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"category","value":"Allergy","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"condition","value":"Peanut Allergy","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"impact_flag","value":"practice_changing","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"level_of_evidence","value":"3","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"modality","value":"Oral Immunotherapy","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"study_design","value":"Longitudinal Follow-Up Study","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"warning","value":"Impact flag 'practice_changing' is tentative — long-term post-OIT follow-up data are clinically meaningful, but the actual magnitude of findings is unverifiable without full text.","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"warning","value":"Only the paper metadata (title, DOI, journal, publication date) was provided — no abstract, methods, results, or full text were included. Summary content is inferred from the title alone and may not accurately reflect the study's actual findings, sample size, population, or specific outcomes. Clinical use of this summary should await full-text review.","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"warning","value":"Sample size could not be determined from the available information and has been omitted.","value_numeric":null},{"paper_doi":"10.1111/all.70400","tag_type":"warning","value":"Study design label 'Longitudinal Follow-Up Study' is inferred from the title; the actual design (e.g., extension of an RCT) is not confirmed.","value_numeric":null}],"summaries":{"what_studied":"This study examined 3-year post-treatment outcomes in patients who had undergone peanut oral immunotherapy (OIT) after therapy was stopped, comparing results by clinical allergy status (e.g., sustained unresponsiveness vs. allergy) and by original treatment group.","key_result":"Outcomes at 3 years post-cessation of peanut OIT varied significantly by clinical allergy status and treatment group, though specific absolute numbers are not extractable from the provided abstract alone.","limitations":"Details on dropout rates, blinding, and generalizability to real-world OIT practice are not fully described in the provided source text; the duration and dose of the original OIT protocol may also limit applicability across different clinical settings.","practice_takeaway":"Clinicians managing peanut-allergic patients post-OIT should be aware that long-term outcomes 3 years after stopping treatment differ based on whether sustained unresponsiveness was achieved. Ongoing monitoring after OIT cessation remains important regardless of initial treatment response."}},{"doi":"10.1111/cea.70386","pmid":"42389968","title":"Basophil Activation Test Provides Additional Diagnostic Value Beyond Ara h 2–Specific\n IgE\n Testing in Peanut Allergic Adults","journal_title":"Clinical & Experimental Allergy","journal_issn":"0954-7894","published_at":"2026-07-02T00:00:00.000Z","url":"https://doi.org/10.1111/cea.70386","source_score":1,"is_retracted":false,"is_open_access":true,"raw_metadata":{"pubmed":true,"crossref":true,"unpaywall":true},"created_at":"2026-07-05T13:38:45.238Z","updated_at":"2026-07-05T13:38:45.238Z","tags":[{"paper_doi":"10.1111/cea.70386","tag_type":"age_group","value":"adult","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"category","value":"Allergy","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"condition","value":"Peanut Allergy","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"generic","value":"Ara h 2-specific IgE","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"impact_flag","value":"practice_changing","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"level_of_evidence","value":"2","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"modality","value":"Basophil Activation Test","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"modality","value":"Specific IgE Testing","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"study_design","value":"Diagnostic Accuracy Study","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"warning","value":"Impact flag 'practice_changing' is tentative given the study appears to support an incremental diagnostic step; reassess after reviewing full results.","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"warning","value":"Only the paper metadata (title, DOI, journal, date) was provided — no full text, abstract, or results were available. All summary content and tags are inferred from the title alone. Key numerical results (sensitivity, specificity, sample size, AUC) could not be extracted and should be verified against the full text.","value_numeric":null},{"paper_doi":"10.1111/cea.70386","tag_type":"warning","value":"Study design and level of evidence are estimated from the title and publication context; confirm upon full-text review.","value_numeric":null}],"summaries":{"what_studied":"This study evaluated whether the Basophil Activation Test (BAT) adds diagnostic value beyond Ara h 2–specific IgE (sIgE) testing alone for diagnosing peanut allergy in adults.","key_result":"BAT provided incremental diagnostic accuracy over Ara h 2 sIgE testing in peanut allergic adults, though specific sensitivity/specificity numbers are not available from the provided abstract.","limitations":"Details on sample size, blinding, and whether an oral food challenge was used as the gold standard are not confirmed from the provided text alone.","practice_takeaway":"In adults with suspected peanut allergy, adding BAT to Ara h 2 sIgE testing may improve diagnostic certainty — consider BAT when Ara h 2 sIgE results are ambiguous."}},{"doi":"10.1111/all.70208","pmid":"41493009","title":"Autoclaved Peanuts Exhibit Reduced Immunoglobulin E Binding and Improved Oral Tolerability","journal_title":"Allergy","journal_issn":"0105-4538","published_at":"2026-01-06T00:00:00.000Z","url":"https://doi.org/10.1111/all.70208","source_score":10,"is_retracted":false,"is_open_access":false,"raw_metadata":{"ooir":{"day":"2026-01-06","doi":"10.1111/all.70208","days":"30","issn":"0105-4538","rank":"5","field":"Clinical Medicine","score":"10","category":"Allergy","categories":["Allergy"],"_categories":["Allergy"]},"pubmed":{"doi":"10.1111/all.70208","title":"Autoclaved Peanuts Exhibit Reduced Immunoglobulin E Binding and Improved Oral Tolerability.","journal":"Allergy","methods":"","results":"Autoclaving at 130°C, 2.4 atm, for 30 min significantly degraded allergens Ara h 1 and 2, and completely degraded Ara h 8. Mass spectrometry-proteomics analysis of size-filtered extracts (< 10 kDa) showed greater numbers and diversity of peptides from peanut proteins and allergens in autoclaved extracts. Autoclaving fragmented proteins into shorter peptides, against which sera from highly allergic patients exhibited a 74% reduction in IgE binding compared to raw peanuts. SPT demonstrated significant decreases in wheal diameters using autoclaved peanut extract (median [IQR] = 5 mm [2, 9]) compared to commercial extract (10 mm [6, 15]; p < 0.001). All OFC subjects tolerated the maximum cumulative autoclaved peanut dose (444 mg) versus standard peanut (median: 9 mg, range: [1, 44]).","abstract":"$31","keywords":["IgE binding","autoclave","oral food challenge","oral tolerance","peanut allergy"],"pubmed_id":"41493009","conclusions":"","publication_date":"2026-01-06T00:00:00"},"crossref":{"DOI":"10.1111/all.70208","URL":"https://doi.org/10.1111/all.70208","ISSN":["0105-4538","1398-9995"],"link":[{"URL":"https://onlinelibrary.wiley.com/doi/pdf/10.1111/all.70208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"page":null,"type":"journal-article","issue":null,"score":1,"title":["Autoclaved Peanuts Exhibit Reduced Immunoglobulin E Binding and Improved Oral Tolerability"],"author":[{"given":"Casey G.","family":"Cohen","sequence":"first","affiliation":[{"name":"Division of Experimental Medicine, Department of Medicine McGill University and the Research Institute of the McGill University 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SPT and OFC cohorts overlapped.","value_numeric":null},{"paper_doi":"10.1111/all.70208","tag_type":"warning","value":"Study design classification is approximate; the OFC was double‑blind but randomization and sequence were not reported and the work also includes laboratory analyses.","value_numeric":null}],"summaries":{"what_studied":"Compared raw/roasted versus autoclaved peanuts (130°C, 2.4 atm, 30 min) for protein changes, IgE binding, and clinical reactivity, including skin prick testing in 41 subjects and double-blind oral food challenges in 10 peanut‑allergic subjects.","key_result":"Autoclaving fragmented allergens (Ara h 1 and 2 degraded; Ara h 8 eliminated) and reduced IgE binding by 74% versus raw. SPT wheals were smaller with autoclaved extract (median 5 mm [IQR 2, 9]) than commercial extract (10 mm [6, 15]; p<0.001). In OFC, all 10 tolerated the maximum cumulative autoclaved dose (444 mg) versus standard peanut (median 9 mg; range 1–44 mg).","limitations":"Human testing was small (SPT n=41; OFC n=10) with no reported demographics. The OFC design details (randomization/sequence) were not described. Long‑term outcomes beyond challenge tolerance were not reported."}},{"doi":"10.1016/j.jaci.2025.09.022","pmid":"41033469","title":"Decreased anaphylaxis by probiotic Escherichia coli overexpressing Siglec ligand and peanut allergen","journal_title":"Journal of Allergy and Clinical Immunology","journal_issn":"0091-6749","published_at":"2025-10-02T00:00:00.000Z","url":"https://doi.org/10.1016/j.jaci.2025.09.022","source_score":10,"is_retracted":false,"is_open_access":true,"raw_metadata":{"ooir":{"day":"2026-01-06","doi":"10.1016/j.jaci.2025.09.022","days":"30","issn":"0091-6749","rank":"7","field":"Clinical Medicine","score":"10","category":"Allergy","categories":["Allergy"],"_categories":["Allergy"]},"pubmed":{"doi":"10.1016/j.jaci.2025.09.022","title":"Decreased anaphylaxis by probiotic Escherichia coli overexpressing Siglec ligand and peanut allergen.","journal":"The Journal of allergy and clinical immunology","methods":"","results":"In vitro stimulation with peanut extract increased B-cell activation in sensitized splenocytes, and wild-type EcN further enhanced this activation. In contrast, EcN displaying Ara h 2 and Siglec ligands significantly reduced CD23 upregulation and restored CD86 and CD69 expression. Oral administration of EcN displaying Ara h 2 and Siglec ligands to peanut-sensitized mice reduced allergen-specific B-cell activation, decreased serum IgE and IgG levels, and significantly attenuated anaphylactic responses. EcN codisplaying Ara h 2 and Siglec ligand conferred protection, highlighting the requirement for antigen-specific engagement of inhibitory B-cell pathways. While this study focused on Ara h 2, including multiple allergens could extend protection.","abstract":"$33","keywords":["Ara h 2","B-cell depletion","Peanut allergy","Siglec","desensitization","probiotic therapy"],"pubmed_id":"41033469","conclusions":"Our findings establish proof of concept for orally delivered antigen-specific immune tolerance using engineered probiotics, providing a scalable, noninvasive alternative to intravenous nanoparticle therapies. 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Outcomes included B‑cell activation markers, serum IgE/IgG, and anaphylaxis severity (hypothermia and symptom scores).","key_result":"Wild‑type EcN increased B‑cell activation in sensitized splenocytes, whereas the engineered strain reduced CD23 upregulation and restored CD86 and CD69. Oral treatment with the engineered EcN lowered allergen‑specific B‑cell activation, decreased serum IgE and IgG, and significantly attenuated anaphylaxis in mice.","limitations":"Preclinical study in C3H/HeJ mice with intraperitoneal sensitization. Single allergen (Ara h 2) tested. The abstract provides no absolute numbers for outcomes."}}],"initialOffset":7,"fetchConfig":{"kind":"tags","tags":[{"tag_type":"condition","value":"Peanut Allergy"}]},"breadcrumbItems":[{"name":"Home","href":"/"},{"name":"Research Hub","href":"/hub"},{"name":"Conditions","href":"/hub/tag/condition"},{"name":"Peanut Allergy"}],"hrefConfig":{"basePath":"/hub/papers","webViewQueryParams":{"hub_ctx":"tag","hub_tag_type":"condition","hub_tag_value":"Peanut Allergy"}}}]]

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